Proteintech, 18502-1-AP, Cytokeratin 17 Polyclonal antibody

Size: 20ul / 150ul The Cytokeratin 17 (18502-1-AP) by Proteintech is a Polyclonal antibody targeting Cytokeratin 17 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 18502-1-AP targets Cytokeratin 17 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, A431 cells Positive IP detected in: HeLa cells Positive IHC detected in: human cervical cancer tissue, human lung cancer tissue, human colon cancer tissue, human pancreas cancer tissue, human skin tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Keratins are a large family of proteins that form the intermediate filaments that make up the cytoskeleton. Type I keratins are a group of acidic intermediate filament proteins and type II keratins are the basic or neutral counterparts. Keratin 17 is encoded by the KRT17 gene and is a type I cytokeratin found in nail beds, hair follicles, sebaceous glands, and epidermal appendages.What is the molecular weight of cytokeratin 17?The molecular weight of cytokeratin 17 is approximately 49 kDa.What is the function of cytokeratin 17?Cytokeratin 17 plays a role in the formation and maintenance of epidermal appendages, especially in determining the shape and orientation of hair. It is required for the maintenance of the anagen (growth) state of hair follicles by modulating the function of TNF-alpha for hair cycling and is also involved in tissue repair (PMID: 16702408).What is the cellular localization of cytokeratin 17?Cytokeratin 17 expression is located in the cytoplasm.What is the tissue specificity of cytokeratin 17?Cytokeratin 17 expression is found in the outer root sheath and medulla regions of the hair follicle, particularly in the eyebrow, beard, nail bed epithelium, basal cells of the oral epithelium, and sweat and mammary glands (PMID: 2451629). Expression can also be found in epithelial layers of the prostate and urinary bladder and cambial cells of the sebaceous gland at the protein level.What is the role of cytokeratin 17 in disease?Mutations in the KRT17 gene are a cause of Jackson-Lawler type pachyonychia congenita type 2 (PC2); an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy (PMID: 7539673). Defects in KRT17 also lead to steatocystoma multiplex (SM) (PMID: 9767294). Cytokeratin 17 acts as an autoantigen in the immunopathogenesis of psoriasis (PMID: 23722817). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13351 Product name: Recombinant human KRT17 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-432 aa of BC011901 Sequence: MTTSIRQFTSSSSIKGSSGLGGGSSRTSCRLSGGLGAGSCRLGSAGGLGSTLGGSSYSSCYSFGSGGGYGSSFGGVDGLLAGGEKATMQNLNDRLASYLDKVRALEEANTELEVKIRDWYQRQAPGPARDYSQYYRTIEELQNKILTATVDNANILLQIDNARLAADDFRTKFETEQALRLSVEADINGLRRVLDELTLARADLEMQIENLKEELAYLKKNHEEEMNALRGQVGGEINVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEDWFFSKTEELNREVATNSELVQSGKSEISELRRTMQALEIELQSQLSMKASLEGNLAETENRYCVQLSQIQGLIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLTQYKKEPVTTRQVRTIVEEVQDGKVISSREQVHQTTR Predict reactive species Full Name: keratin 17 Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 48 kDa GenBank Accession Number: BC011901 Gene Symbol: Cytokeratin 17 Gene ID (NCBI): 3872 RRID: AB_10644296 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q04695 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.