Proteintech, 18714-1-AP, VWA2 Polyclonal antibody

Size: 20ul / 150ul The VWA2 (18714-1-AP) by Proteintech is a Polyclonal antibody targeting VWA2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 18714-1-AP targets VWA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A375 cells Positive IHC detected in: human colon tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells, A375 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information VWA2, also named as AMACO and CCSP2, is a novel candidate serological marker of colon neoplasia. VWA2 is found in the media of variesy of established cell lines of both fibroblast and epithelial origin. This antibody can recognize all the 3 isoforms of VWA2. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: von Willebrand factor A domain containing 2 Calculated Molecular Weight: 82 kDa Observed Molecular Weight: 85 kDa GenBank Accession Number: BC128588 Gene Symbol: VWA2 Gene ID (NCBI): 340706 RRID: AB_10598772 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q5GFL6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.