Proteintech, 18724-1-AP, GABARAPL2-Specific Polyclonal antibody

Size: 20ul / 150ul The GABARAPL2-Specific (18724-1-AP) by Proteintech is a Polyclonal antibody targeting GABARAPL2-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 18724-1-AP targets GABARAPL2-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: COLO 320 cells, human brain tissue, mouse brain tissue, mouse spleen tissue, PC-3 cells Positive IHC detected in: human heart tissue, human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Chloroquine treated HepG2 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information GABARAPL2 (also known as Golgi-associated ATPase enhancer of 16 kDa; GATE-16) was originally characterized as a factor essential for intra-Golgi protein transport. Later GABARAPL2 along with LC3 and GABARAP, were identified as the homologs of yeast ATG8 which is a unique ubiquitin-like protein essential for autophagy. When induced GABARAPL2 is first digested by ATG4 family enzymes to become its mature form: GABARAPL2-I, later conjugated to PE lipid as lipidated form: GABARAPL2-II. The lipidated form migrates faster than its mature form on SDS-PAGE gel. GABARAPL2 is strongly expressed in the brain. This antibody is specific to GABARAPL2. It has no cross-reaction to other GABARAP protein. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: GABA(A) receptor-associated protein-like 2 Calculated Molecular Weight: 14 kDa Observed Molecular Weight: 14 kDa GenBank Accession Number: BC005985 Gene Symbol: GABARAPL2 Gene ID (NCBI): 11345 RRID: AB_2232116 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P60520 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.