Proteintech, 18799-1-AP, PSME4 Polyclonal antibody

Size: 20ul / 150ul The PSME4 (18799-1-AP) by Proteintech is a Polyclonal antibody targeting PSME4 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 18799-1-AP targets PSME4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HepG2 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, Hela cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information PSME4, like PSME1, 2, and 3, encodes proteasome activator complex subunit 4 also named proteasome activator 200 kDa (PA200). PA200 activates proteasomal cleavage of peptides in an energy-independent manner. After exposure of cells to ionizing radiation, PA200 forms nuclear foci and enhances proteasome activity independent of the stage of cell cycle arrest. PA200 also may be involved in spermatogenesis. The antibody is expected to recognize all the 4 isoforms of PSME4 but appears to mainly detect isoform 2 of 140-kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: proteasome (prosome, macropain) activator subunit 4 Calculated Molecular Weight: 211 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: BC113668 Gene Symbol: PSME4 Gene ID (NCBI): 23198 RRID: AB_10598020 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14997 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.