Proteintech, 18825-1-AP, RBP2 Polyclonal antibody

Size: 20ul / 150ul The RBP2 (18825-1-AP) by Proteintech is a Polyclonal antibody targeting RBP2 in WB, IF/ICC, IF-P, IP, ELISA applications with reactivity to human, mouse, rat samples 18825-1-AP targets RBP2 in WB, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293T cells Positive IP detected in: HeLa cells Positive IF-P detected in: mouse small intestine tissue Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information KDM5A, also named as JARID1A, RBBP2 and RBP2, belongs to the JARID1 histone demethylase family. KDM5A is a Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. RBP2 may stimulate transcription mediated by nuclear receptors. It may be involved in transcriptional regulation of Hox proteins during cell differentiation. It may participate in transcriptional repression of cytokines such as CXCL12. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: lysine (K)-specific demethylase 5A Calculated Molecular Weight: 192 kDa Observed Molecular Weight: 192 kDa GenBank Accession Number: NM_005056 Gene Symbol: KDM5A Gene ID (NCBI): 5927 RRID: AB_10638436 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P29375 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.