Proteintech, 18895-1-AP, LAPTM4B Polyclonal antibody

Size: 20ul / 150ul The LAPTM4B (18895-1-AP) by Proteintech is a Polyclonal antibody targeting LAPTM4B in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 18895-1-AP targets LAPTM4B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse lymph tissue, rat lymph tissue Positive IHC detected in: human liver cancer tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information LAPTM4B, a novel oncoprotein, is associated with the prognosis of cancer, and may play important roles in the initiation, promotion and metastasis of tumors. It was initially identified as a hepatocellular carcinoma-associated gene, and was recently shown to be up-regulated in various human cancers. Some studies indicated that LAPTM4B may be an useful marker of prognosis in ovarian carcinoma and endometrial cancer. LAPTM4B belongs to the LAPTM4/LAPTM5 transporter family. It has 3 isoforms with molecular masses 41 kDa, 35 kDa and 24 kDa. This antibody can recognize all the 3 isoforms of LAPTM4B. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13521 Product name: Recombinant human LAPTM4B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 31-317 aa of BC014129 Sequence: TDPAEARSSRGIEEAGPRAHGRAGREPERRRSRQQRRGGLQARRSTLLKTCARARATAPGAMKMVAPWTRFYSNSCCLCCHVRTGTILLGVWYLIINAVVLLILLSALADPDQYNFSSSELGGDFEFMDDANMCIAIAISLLMILICAMATYGAYKQRAAWIIPFFCYQIFDFALNMLVAITVLIYPNSIQEYIRQLPPNFPYRDDVMSVNPTCLVLIILLFISIILTFKGYLISCVWNCYRYINGRNSSDVLVYVTSNDTTVLLPPYDDATVNGAAKEPPPPYVSA Predict reactive species Full Name: lysosomal protein transmembrane 4 beta Calculated Molecular Weight: 41 kDa Observed Molecular Weight: 24 kDa GenBank Accession Number: BC014129 Gene Symbol: LAPTM4B Gene ID (NCBI): 55353 RRID: AB_10597385 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86VI4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.