Proteintech, 18943-1-AP, MYOD1 Polyclonal antibody

Size: 20ul / 150ul The MYOD1 (18943-1-AP) by Proteintech is a Polyclonal antibody targeting MYOD1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 18943-1-AP targets MYOD1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, human heart tissue, rat heart tissue Positive IHC detected in: mouse embryo tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, rabbit, chicken, zebrafish, sheep, goat, duck Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13512 Product name: Recombinant human MYOD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-320 aa of BC064493 Sequence: MELLSPPLRDVDLTAPDGSLCSFATTDDFYDDPCFDSPDLRFFEDLDPRLMHVGALLKPEEHSHFPAAVHPAPGAREDEHVRAPSGHHQAGRCLLWACKACKRKTTNADRRKAATMRERRRLSKVNEAFETLKRCTSSNPNQRLPKVEILRNAIRYIEGLQALLRDQDAAPPGAAAAFYAPGPLPPGRGGEHYSGDSDASSPRSNCSDGMMDYSGPPSGARRRNCYEGAYYNEAPSEPRPGKSAAVSSLDCLSSIVERISTESPAAPALLLADVPSESPPRRQEAAAPSEGESSGDPTQSPDAAPQCPAGANPNPIYQVL Predict reactive species Full Name: myogenic differentiation 1 Calculated Molecular Weight: 320 aa, 35 kDa Observed Molecular Weight: 35-45 kDa GenBank Accession Number: BC064493 Gene Symbol: MYOD1 Gene ID (NCBI): 4654 RRID: AB_10603467 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P15172 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.