Proteintech, 19055-1-AP, Aiolos/IKZF3 Polyclonal antibody

Size: 20ul / 150ul The Aiolos/IKZF3 (19055-1-AP) by Proteintech is a Polyclonal antibody targeting Aiolos/IKZF3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 19055-1-AP targets Aiolos/IKZF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, Jurkat cells Positive IHC detected in: human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information IKZF3, also named as Zinc finger protein Aiolos or ZNFN1A3, is a 509 amino acid protein, which contains 6 C2H2-type zinc fingers and belongs to the Ikaros C2H2-type zinc-finger protein family. IKZF3 localizes in the nucleus and cytoplasm. IKZF3 can form homodimer, and heterodimer with other IKAROS family members. IKZF3 is expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus. IKZF3 as a transcription factor plays an important role in the regulation of lymphocyte differentiation. The calculated molecular weight of IKZF3 is 58 kDa, but the modified IKZF3 protein is about 65-70 kDa. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5537 Product name: Recombinant human IKZF3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 209-509 aa of BC032707 Sequence: RSYKQRSSLEEHKERCRTFLQSTDPGDTASAEARHIKAEMGSERALVLDRLASNVAKRKSSMPQKFIGEKRHCFDVNYNSSYMYEKESELIQTRMMDQAINNAISYLGAEALRPLVQTPPAPTSEMVPVISSMYPIALTRAEMSNGAPQELEKKSIHLPEKSVPSERGLSPNNSGHDSTDTDSNHEERQNHIYQQNHMVLSRARNGMPLLKEVPRSYELLKPPPICPRDSVKVINKEGEVMDVYRCDHCRVLFLDYVMFTIHMGCHGFRDPFECNMCGYRSHDRYEFSSHIARGEHRALLK Predict reactive species Full Name: IKAROS family zinc finger 3 (Aiolos) Calculated Molecular Weight: 509 aa, 58 kDa Observed Molecular Weight: 58-70 kDa GenBank Accession Number: BC032707 Gene Symbol: IKZF3 Gene ID (NCBI): 22806 RRID: AB_10643381 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UKT9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.