Proteintech, 19177-1-AP, SFRP5 Polyclonal antibody

Size: 20ul / 150ul The SFRP5 (19177-1-AP) by Proteintech is a Polyclonal antibody targeting SFRP5 in IHC, ELISA applications with reactivity to human samples 19177-1-AP targets SFRP5 in IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: human cervical cancer tissue, human thyroid cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Secreted frizzled-related protein 5 (SFRP5) is an anti-inflammatory adipokine whose expression is perturbed in models of obesity and type 2 diabetes. SFRP5 is secreted by adipocytes and that it controls the micro-environment of white adipose tissue under conditions of metabolic stress (PMID: 20558665). SFRP5 gene consists of 317 amino acid residues and 3 coding exons and maps to chromosome 10q24.1 in humans. SFRP5 was initially found to be highly expressed in retinal pigment epithelium cells and moderately expressed in the pancreas. SFRP5 is involved in the pathological process of obesity, insulin resistance, dyslipidemia, inflammation, and fibrosis and is associated with obesity, T2D, and ASCVD (PMID: 31199714, PMID: 32004418). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5715 Product name: Recombinant human SFRP5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-317 aa of BC050435 Sequence: MRAAAAAGGVRTAALALLLGALHWAPARCEEYDYYGWQAEPLHGRSYSKPPQCLDIPADLPLCHTVGYKRMRLPNLLEHESLAEVKQQASSWLPLLAKRCHSDTQVFLCSLFAPVCLDRPIYPCRSLCEAVRAGCAPLMEAYGFPWPEMLHCHKFPLDNDLCIAVQFGHLPATAPPVTKICAQCEMEHSADGLMEQMCSSDFVVKMRIKEIKIENGDRKLIGAQKKKKLLKPGPLKRKDTKRLVLHMKNGAGCPCPQLDSLAGSFLVMGRKVDGQLLLMAVYRWDKKNKEMKFAVKFMFSYPCSLYYPFFYGAAEPH Predict reactive species Full Name: secreted frizzled-related protein 5 Calculated Molecular Weight: 36 kDa GenBank Accession Number: BC050435 Gene Symbol: SFRP5 Gene ID (NCBI): 6425 RRID: AB_3085575 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q5T4F7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.