Proteintech, 19221-1-AP, DNMT2 Polyclonal antibody

Size: 20ul / 150ul The DNMT2 (19221-1-AP) by Proteintech is a Polyclonal antibody targeting DNMT2 in WB, ELISA applications with reactivity to human, mouse, rat samples 19221-1-AP targets DNMT2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, mouse liver tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Dnmt2 was initially assigned a member of the DNA methyltransferase family on the basis of its extensive homology with eukaryotic and prokaryotic DNA-(cytosine C5)-methyltransferases . However, in the apparent absence of a phenotype in dnmt2 knockout cells, Dnmt2's possible biological function remained unknown , even though Dnmt2 is strongly conserved and it is found in species ranging from Schizosaccharomyces pombe to human. Later very weak, residual DNA methylation activity was found with enzymes from different species. The finding that Dnmt2 is an active RNA methyltransferase capable of methylating the C38 position of the tRNA Asp came as a surprise . However, still no cellular function of the tRNA Asp methylation has been found, although in Zebrafish Dnmt2 knock-down caused a developmental phenotype . It is very intriguing that an enzyme that looks like a DNA methyltransferase can methylate RNA, in particular since the RNA and DNA specific m5 C methyltransferases use different catalytic residues and a different mechanism for the methyl transfer reaction . the predicted molecular weight of Dnmt2 is 45 kDa, but it may migrate to the 40 kDa (PMID: 18567810 ) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5571 Product name: Recombinant human DNMT2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-391 aa of BC047733 Sequence: MEPLRVLELYSGVGGMHHALRESCIPAQVVAAIDVNTVANEVYKYNFPHTQLLAKTIEGITLEEFDRLSFDMILMSPPCQPFTRIGRQGDMTDSRTNSFLYILDILPRLQKLPKYILLENVKGFEVSSTRDLLIQTIENCGFQYQEFLLSPTSLGIPNSRLRYFLIAKLQSEPLPFQAPGQVLMEFPKIESVHPQKYAMDVENKIQEKNVEPNISFDGSIQCSGKDAILFKLETAEEIHRKNQQDSDLSVKMLKDFLEDDTDVNQYLLPPKSLLRYALLLDIVQPTCRRSVCFTKGYGSYIEGTGSVLQTAEDVQVENIYKSLTNLSQEEQITKLLILKLRYFTPKEIANLLGFPPEFGFPEKITVKQRYRLLGNSLNVHVVAKLIKILYE Predict reactive species Full Name: tRNA aspartic acid methyltransferase 1 Calculated Molecular Weight: 45 kDa Observed Molecular Weight: 43-45 kDa GenBank Accession Number: BC047733 Gene Symbol: DNMT2 Gene ID (NCBI): 1787 RRID: AB_10597075 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O14717 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.