Proteintech, 19245-1-AP, KIF18A Polyclonal antibody

Size: 20ul / 150ul The KIF18A (19245-1-AP) by Proteintech is a Polyclonal antibody targeting KIF18A in WB, IHC, ELISA applications with reactivity to human samples 19245-1-AP targets KIF18A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, HeLa cells Positive IHC detected in: human colon cancer, human breast cancer tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information KIF18A belongs to the Kinesin family. KIF18A binds to microtubules and moves along them using energy from ATP hydrolysis. KIF18A is related to the localization of membrane organelles and protein complexes, as well as the maintenance of microtubule dynamics and mitosis. KIF18A expression is low in normal tissues and high in solid tumors, including breast cancer, hepatocellular carcinoma and lung adenocarcinoma, colorectal cancer, renal cell carcinoma, and head and neck squamous cell carcinoma. The molecular weight of KIF18A is 102 kDa (PMID: 36830695). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6166 Product name: Recombinant human KIF18A protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-279 aa of BC048347 Sequence: MSVTEEDLCHHMKVVVRVRPENTKEKAAGFHKVVHVVDKHILVFDPKQEEVSFFHGKKTTNQNVIKKQNKDLKFVFDAVFDETSTQSEVFEHTTKPILRSFLNGYNCTVLAYGATGAGKTHTMLGSADEPGVMYLTMLHLYKCMDEIKEEKICSTAVSYLEVYNEQIRDLLVNSGPLAVREDTQKGVVVHGLTLHQPKSSEEILHLLDNGNKNRTQHPTDMNATSSRSHAVFQIYLRQQDKTASINQNVRIAKMSLIDLAGSERASTSGAKGTRFVEGT Predict reactive species Full Name: kinesin family member 18A Calculated Molecular Weight: 102 kDa Observed Molecular Weight: 102 kDa GenBank Accession Number: BC048347 Gene Symbol: KIF18A Gene ID (NCBI): 81930 RRID: AB_10598464 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8NI77 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.