Proteintech, 19401-1-AP, B3GAT1 Polyclonal antibody

Size: 20ul / 150ul The B3GAT1 (19401-1-AP) by Proteintech is a Polyclonal antibody targeting B3GAT1 in WB, IHC, ELISA applications with reactivity to human, mouse samples 19401-1-AP targets B3GAT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: PC-12 cells, SH-SY5Y cells Positive IHC detected in: mouse brain tissue, mouse spleen tissue, human thyroid cancer tissue, human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 19401-1-AP antibody detectes 45 and 70 kDa forms in SDS-PAGE which might as a result of different degrees of glycosylation or the formation of homodimer. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13550 Product name: Recombinant human CD57 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 25-334 aa of BC010466 Sequence: HQSTLAPLLAVHKDEGSDPRRETPPGADPREYCTSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSSQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPSVEI Predict reactive species Full Name: beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) Calculated Molecular Weight: 38 kDa Observed Molecular Weight: 45 kDa, 70 kDa GenBank Accession Number: BC010466 Gene Symbol: B3GAT1 Gene ID (NCBI): 27087 RRID: AB_2878577 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9P2W7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.