Proteintech, 19493-1-AP, VLDLR Polyclonal antibody

Size: 20ul / 150ul The VLDLR (19493-1-AP) by Proteintech is a Polyclonal antibody targeting VLDLR in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 19493-1-AP targets VLDLR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HuH-7 cells, MCF-7 cells, MDA-MB-231 cells Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information VLDLR binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. VLDLR binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation (cytoplasmic domain of low-density lipoprotein receptor). For glycosylated, the MW of VLDLR migrates to ~160kd. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, chicken, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: very low density lipoprotein receptor Calculated Molecular Weight: 96 kDa Observed Molecular Weight: 96 kDa GenBank Accession Number: NM_003383 Gene Symbol: VLDLR Gene ID (NCBI): 7436 RRID: AB_10644342 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P98155 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.