Proteintech, 19530-1-AP, GATA4-Specific Polyclonal antibody

Size: 20ul / 150ul The GATA4-Specific (19530-1-AP) by Proteintech is a Polyclonal antibody targeting GATA4-Specific in WB, ELISA applications with reactivity to human, mouse, rat samples 19530-1-AP targets GATA4-Specific in WB, IHC, IF, IP, CoIP, ChiP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, mouse liver tissue, mouse heart tissue, rat heart tissue, PC-3 cells, NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information What is the molecular weight of GATA4? Is GATA4 post-translationally modified?The molecular weight of GATA4 is 48 kDa. GATA4 can be phosphorylated, which regulates its activity (PMID: 10788492).What is the subcellular localization of GATA4?GATA4 localizes to the nucleus. The nuclear localization motif is present in the C-terminal part of GATA4 (PMID: 9079680).What is the tissue expression pattern of GATA4?GATA4 was initially identified as a cardiac transcription factor important for the development of the heart and as one of the earliest transcription factors expressed in developing cardiac cells. However, GATA4 is expressed in numerous organs such as the liver, lung, gonads, and small intestine (PMID: 8455608). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: GATA binding protein 4 Calculated Molecular Weight: 45 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: NM_002052 Gene Symbol: GATA4 Gene ID (NCBI): 2626 RRID: AB_10642003 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P43694 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.