Proteintech, 19540-1-AP, ZIP kinase Polyclonal antibody

Size: 20ul / 150ul The ZIP kinase (19540-1-AP) by Proteintech is a Polyclonal antibody targeting ZIP kinase in IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 19540-1-AP targets ZIP kinase in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive IHC detected in: human stomach cancer tissue, rat colon tissue, rat heart tissue, mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, U-251 cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information Zipper-interacting protein (ZIP) kinase, also known as death-associated protein kinase 3 (DAPK3), is aserine/threonine kinase that positively regulates mitochondrial-dependent apoptosis as well as autophagyand acts as a tumor suppressor.What is the molecular weight of ZIP kinase? Is ZIP kinase post-translationally modified?The molecular weight of ZIP kinase is 52 kDa. ZIP kinase contains seven phosphorylation sites (six threonineand one serine residues) that control its activity and localization (PMID: 24193917).What is the subcellular localization of ZIP kinase?ZIP kinase contains nuclear localization signal in its C-terminal part.ZIP kinase is present both in the cytoplasmand in the nucleus. In the cytoplasm ZIP kinase interacts with actin cytoskeleton, regulating contraction ofsmooth muscles (PMID: 16005610) and membrane blebbing (PMID: 11781833). In the nucleus it interactswith several transcription factors regulating gene expression.What is the tissue expression pattern of ZIP kinase?ZIP kinase is ubiquitously expressed. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: death-associated protein kinase 3 Calculated Molecular Weight: 53 kDa Observed Molecular Weight: 37, 52 kDa GenBank Accession Number: NM_001348 Gene Symbol: DAPK3 Gene ID (NCBI): 1613 RRID: AB_10860106 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O43293 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.