Proteintech, 19544-1-AP, FEM1B Polyclonal antibody

Size: 20ul / 150ul The FEM1B (19544-1-AP) by Proteintech is a Polyclonal antibody targeting FEM1B in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 19544-1-AP targets FEM1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: PC-3 cells, mouse pancreas tissue, mouse testis tissue Positive IHC detected in: human pancreas cancer tissue, mouse pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: PC-3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information FEM1B, also named F1AA and KIAA0396, belongs to the fem-1 family. FEM1B is a component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. FEM1B is involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. It is involved in glucose homeostasis in pancreatic islets. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: fem-1 homolog b (C. elegans) Calculated Molecular Weight: 70 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: NM_015322 Gene Symbol: FEM1B Gene ID (NCBI): 10116 RRID: AB_10644294 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UK73 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.