Proteintech, 19563-1-AP, UBASH3B Polyclonal antibody

Size: 20ul / 150ul The UBASH3B (19563-1-AP) by Proteintech is a Polyclonal antibody targeting UBASH3B in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 19563-1-AP targets UBASH3B in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, mouse lung tissue Positive IP detected in: mouse kidney tissue Positive IHC detected in: human hepatocirrhosis tissue, human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information UBASH3B, also named STS1 or TULA-2, is a protein that contains a ubiquitin-associated domain at the N-terminus, a SH3 domain, and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. TULA-2 is supposed to form homodimer and interact with ubiquitin and mono-ubiquitinated proteins. TULA-2 functions to promote accumulation of activated target receptors, such as T-cell receptors and EGFR on the cell surface. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ubiquitin associated and SH3 domain containing, B Calculated Molecular Weight: 73 kDa Observed Molecular Weight: 66-70 kDa GenBank Accession Number: NM_032873 Gene Symbol: UBASH3B Gene ID (NCBI): 84959 RRID: AB_10643379 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8TF42 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.