Proteintech, 19690-1-AP, EIF2C1-Specific Polyclonal antibody

Size: 20ul / 150ul The EIF2C1-Specific (19690-1-AP) by Proteintech is a Polyclonal antibody targeting EIF2C1-Specific in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 19690-1-AP targets EIF2C1-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells Positive IHC detected in: human cervical cancer tissue, human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information EIF2C1, also named as AGO1, belongs to the argonaute family and Ago subfamily. EIF2C1 is required for RNA-mediated gene silencing (RNAi). It binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. EIF2C1 may also be required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). The antibody is specific to EIF2C1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: eukaryotic translation initiation factor 2C, 1 Calculated Molecular Weight: 97 kDa Observed Molecular Weight: 97 kDa GenBank Accession Number: NM_012199 Gene Symbol: EIF2C1 Gene ID (NCBI): 26523 RRID: AB_10863657 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UL18 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.