Proteintech, 19730-1-AP, Tenascin-R Polyclonal antibody

Size: 20ul / 150ul The Tenascin-R (19730-1-AP) by Proteintech is a Polyclonal antibody targeting Tenascin-R in WB, IHC, ELISA applications with reactivity to human samples 19730-1-AP targets Tenascin-R in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human brain tissue, SH-SY5Y cells Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information TNR, also named as Restrictin and Janusin, belongs to the tenascin family. Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. These interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides, TNR inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides, TNR results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1, TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B, TNR may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediate inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance. The antibody is specific to TNR. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: tenascin R (restrictin, janusin) Calculated Molecular Weight: 150 kDa Observed Molecular Weight: 180 kDa, 160 kDa GenBank Accession Number: NM_003285 Gene Symbol: TNR Gene ID (NCBI): 7143 RRID: AB_10665365 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q92752 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.