Proteintech, 19740-1-AP, KALRN Polyclonal antibody

Size: 20ul / 150ul The KALRN (19740-1-AP) by Proteintech is a Polyclonal antibody targeting KALRN in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 19740-1-AP targets KALRN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse colon tissue, human kidney tissue, human brain tissue, rat colon tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information KALRN, also named as DUET, DUO, HAPIP and TRAD, belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. KALRN promotes the exchange of GDP by GTP. KALRN activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. KALRN induces lamellipodia independent of its GEF activity. The antibody can recognize isoform1 and isoform2 of KALRN. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: kalirin, RhoGEF kinase Calculated Molecular Weight: 340 kDa Observed Molecular Weight: 200 kDa GenBank Accession Number: NM_007064 Gene Symbol: KALRN Gene ID (NCBI): 8997 RRID: AB_10638319 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60229 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.