Proteintech, 19771-1-AP, NLRP3 Polyclonal antibody

Size: 20ul / 150ul The NLRP3 (19771-1-AP) by Proteintech is a Polyclonal antibody targeting NLRP3 in WB, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human samples 19771-1-AP targets NLRP3 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human samples. Tested Applications Positive WB detected in: THP-1 cells, Raji cells, LPS treated THP-1 cells Positive IP detected in: THP-1 cells Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: THP-1 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information NALP3, also named C1orf7, CIAS1, and PYPAF1, belongs to the NLRP family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NALP3 may function as an inducer of apoptosis. It interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling.NALP3 inhibits TNF-alpha-induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. NALP3 activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) which is also known as familial cold urticaria. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) which is urticaria-deafness-amyloidosis syndrome. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) which is also known as neonatal onset multisystem inflammatory disease (NOMID). The antibody recognizes the C-term of NALP3. Specification Tested Reactivity: human Cited Reactivity: human, pig, bovine, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: NLR family, pyrin domain containing 3 Calculated Molecular Weight: 118 kDa Observed Molecular Weight: 110 kDa GenBank Accession Number: NM_001127461 Gene Symbol: NLRP3 Gene ID (NCBI): 114548 RRID: AB_10646484 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96P20 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.