Proteintech, 19841-1-AP, KTN1 Polyclonal antibody

Size: 20ul / 150ul The KTN1 (19841-1-AP) by Proteintech is a Polyclonal antibody targeting KTN1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 19841-1-AP targets KTN1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, A549 cells, Jurkat cells, K-562 cells, PC-3 cells, SKOV-3 cells Positive IP detected in: Jurkat cells Positive IHC detected in: human ovary tumor tissue, human bowen disease, human colon tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information KTN1/Kinectin is an integral endoplasmic reticulum (ER) membrane protein which acts as dynamic anchor to facilitate ER membrane extension along microtubules. It has been found to bind kinesin, Rho GTPase, and translation elongation factor-1delta and gets involved in organelle motility and protein synthesis. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13854 Product name: Recombinant human KTN1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 951-1306 aa of BC117132 Sequence: KSQIEQLKQQNYQQASSFPPHEELLKVISEREKEISGLWNELDSLKDAVEHQRKKNNDLREKNWEAMEALASTEKMLQDKVNKTSKERQQQVEAVELEAKEVLKKLFPKVSVPSNLSYGEWLHGFEKKAKECMAGTSGSEEVKVLEHKLKEADEMHTLLQLECEKYKSVLAETEGILQKLQRSVEQEENKWKVKVDESHKTIKQMQSSFTSSEQELERLRSENKDIENLRREREHLEMELEKAEMERSTYVTEVRELKAQLNETLTKLRTEQNERQKVAGDLHKAQQSLELIQSKIVKAAGDTTVIENSDVSPETESSEKETMSVSLNQTVTQLQQLLQAVNQQLTKEKEHYQVLE Predict reactive species Full Name: kinectin 1 (kinesin receptor) Calculated Molecular Weight: 1357 aa, 156 kDa Observed Molecular Weight: 156 kDa GenBank Accession Number: BC117132 Gene Symbol: KTN1 Gene ID (NCBI): 3895 RRID: AB_10642436 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86UP2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.