Proteintech, 20011-1-AP, CYP4F8 Polyclonal antibody

Size: 20ul / 150ul The CYP4F8 (20011-1-AP) by Proteintech is a Polyclonal antibody targeting CYP4F8 in IHC, ELISA applications with reactivity to human samples 20011-1-AP targets CYP4F8 in IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: human skin tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information CYP4F8, also named as CPF8, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. CYP4F8 hydroxylates arachidonic acid (20:4n-6) to (18R)-hydroxyarachidonate. CYP4F8 shows little activity against prostaglandin (PG) D2, PGE1, PGE2, PGF2alpha, and leukotriene B4. CYP4F8 catalyzes omega-2 and omega-3-hydroxylation of PGH1 and PGH2. Catalyzes epoxidation of 4,7,10,13,16,19-(Z)-docosahexaenoic acid (22:6n-3) and 7,10,13,16,19-(Z)-docosapentaenoic acid (22:5n-3) and omega-3-hydroxylation of 4,7,10,13,16-(Z)-docosapentaenoic acid (22:5n-6). It catalyzes hydrxylation of PGI2 and carbaprostacyclin. The antibody has no cross reaction to other CYP4F member Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cytochrome P450, family 4, subfamily F, polypeptide 8 Calculated Molecular Weight: 60 kDa GenBank Accession Number: NM_007253 Gene Symbol: CYP4F8 Gene ID (NCBI): 11283 RRID: AB_2878634 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P98187 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.