Proteintech, 20074-1-AP, GPR101-Specific Polyclonal antibody

Size: 20ul / 150ul The GPR101-Specific (20074-1-AP) by Proteintech is a Polyclonal antibody targeting GPR101-Specific in WB, IHC, FC (Intra), ELISA applications with reactivity to human, monkey samples 20074-1-AP targets GPR101-Specific in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, monkey samples. Tested Applications Positive WB detected in: HEK-293 cells, COS-7 cells Positive IHC detected in: mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information GPR101, also named as GPCR6, belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. This antibody is specific to GPR101. Specification Tested Reactivity: human, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: G protein-coupled receptor 101 Calculated Molecular Weight: 57 kDa Observed Molecular Weight: 57 kDa GenBank Accession Number: NM_054021 Gene Symbol: GPR101 Gene ID (NCBI): 83550 RRID: AB_10665356 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96P66 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.