Proteintech, 20235-1-AP, NTN1-Specific Polyclonal antibody

Size: 20ul / 150ul The NTN1-Specific (20235-1-AP) by Proteintech is a Polyclonal antibody targeting NTN1-Specific in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 20235-1-AP targets NTN1-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, mouse brain tissue, rat heart tissue, rat kidney tissue Positive IHC detected in: mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information NTN1 is also named as NTN1L. Netrins control the guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serves as a survival factor through its association with its receptors, which prevents the initiation of apoptosis. NTN1 is involved in colorectal tumorigenesis by regulating apoptosis. 20235-1-AP antibody detects 65-68 kDa (3 kDa signal peptide) protein in SDS-PAGE. This antibody is specific to NTN1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: netrin 1 Calculated Molecular Weight: 68 kDa Observed Molecular Weight: 65-68 kDa GenBank Accession Number: NM_004822 Gene Symbol: NTN1 Gene ID (NCBI): 9423 RRID: AB_2918065 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95631 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.