Proteintech, 20364-1-AP, RBAP48 Polyclonal antibody

Size: 20ul / 150ul The RBAP48 (20364-1-AP) by Proteintech is a Polyclonal antibody targeting RBAP48 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 20364-1-AP targets RBAP48 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A431 cells, L02 cells, Jurkat cells, HepG2 cells, K-562 cells, HeLa cells, human brain tissue, HEK-293 cells, SH-SY5Y cells, mouse brain tissue Positive IP detected in: HeLa cells Positive IHC detected in: human cervical cancer tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information RBBP4, also named as RBAP48 and CAF-I p48, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP4 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP4. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: retinoblastoma binding protein 4 Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 48-50 kDa GenBank Accession Number: NM_005610 Gene Symbol: RBBP4 Gene ID (NCBI): 5928 RRID: AB_10694151 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q09028 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.