Proteintech, 20365-1-AP, RbAp46 Polyclonal antibody

Size: 20ul / 150ul The RbAp46 (20365-1-AP) by Proteintech is a Polyclonal antibody targeting RbAp46 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 20365-1-AP targets RbAp46 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, COS-7 cells, HEK-293T cells, HeLa cells Positive IP detected in: A431 cells Positive IHC detected in: human breast cancer tissue, human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information RBBP7, also named as RBAP46, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP7 is a core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP7. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: retinoblastoma binding protein 7 Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 52 kDa GenBank Accession Number: NM_002893 Gene Symbol: RBBP7 Gene ID (NCBI): 5931 RRID: AB_10697660 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q16576 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.