Proteintech, 20370-1-AP, POLR2B-Specific Polyclonal antibody

Size: 20ul / 150ul The POLR2B-Specific (20370-1-AP) by Proteintech is a Polyclonal antibody targeting POLR2B-Specific in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 20370-1-AP targets POLR2B-Specific in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, HeLa cells Positive IP detected in: HeLa cells Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information POLR2B belongs to the RNA polymerase beta chain family. It is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is the second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. POLR2B proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. The antibody is specific to POLR2B. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: polymerase (RNA) II (DNA directed) polypeptide B, 140kDa Calculated Molecular Weight: 134 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: NM_000938 Gene Symbol: POLR2B Gene ID (NCBI): 5431 RRID: AB_10693617 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P30876 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.