Proteintech, 20422-1-AP, B3GNT5 Polyclonal antibody

Size: 20ul / 150ul The B3GNT5 (20422-1-AP) by Proteintech is a Polyclonal antibody targeting B3GNT5 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 20422-1-AP targets B3GNT5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse lung tissue, MCF-7 cells Positive IHC detected in: human lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information B3GNT5 (UDP-GlcNAc:β-Gal β-1,3-N-acetylglucosaminyltransferase 5) is a glycosyltransferase that transfers an N-acetylglucosamine (GlcNAc) from uridine diphosphate (UDP)-GlcNAc to galactose at the nonreducing end of carbohydrate chain. B3GNT5 is required for tumorigenicity of breast cancer and glycosylation of B3GNT5 is critical for its protein stabilization (PMID: 35526049). B3GNT5 was highly expressed in human GBM and glioma cell lines in culture, and was associated with poor patient survival (PMID: 32677340). B3GNT5 was N-glycosylated, and glycosylation of B3GNT5 stabilized its protein for non-glycosylated B3GNT5 protein degraded more rapidly than glycosylated form (PMID: 35526049). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14246 Product name: Recombinant human B3GNT5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 36-378 aa of BC028058 Sequence: DNHIVSHMKSYSYRYLINSYDFVNDTLSLKHTSAGPRYQYLINHKEKCQAQDVLLLLFVKTAPENYDRRSGIRRTWGNENYVRSQLNANIKTLFALGTPNPLEGEELQRKLAWEDQRYNDIIQQDFVDSFYNLTLKLLMQFSWANTYCPHAKFLMTADDDIFIHMPNLIEYLQSLEQIGVQDFWIGRVHRGAPPIRDKSSKYYVSYEMYQWPAYPDYTAGAAYVISGDVAAKVYEASQTLNSSLYIDDVFMGLCANKIGIVPQDHVFFSGEGKTPYHPCIYEKMMTSHGHLEDLQDLWKNATDPKVKTISKGFFGQIYCRLMKIILLCKISYVDTYPCRAAFI Predict reactive species Full Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 Calculated Molecular Weight: 378 aa, 44 kDa Observed Molecular Weight: 44 kDa GenBank Accession Number: BC028058 Gene Symbol: B3GNT5 Gene ID (NCBI): 84002 RRID: AB_10694280 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BYG0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.