Proteintech, 20443-1-AP, Bestrophin-3 Polyclonal antibody

Size: 20ul / 150ul The Bestrophin-3 (20443-1-AP) by Proteintech is a Polyclonal antibody targeting Bestrophin-3 in WB, IHC, ELISA applications with reactivity to human, mouse samples 20443-1-AP targets Bestrophin-3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse colon tissue Positive IHC detected in: human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Bestrophin-3 (BEST3) is a member of the Bestrophin (VMD2) Ca2+-activated Cl channel gene family. Bestrophin-3 is involved in the regulation of cell proliferation, apoptosis and differentiation of a variety of physiological functions (PMID: 25329324). Human BEST3 gene is located at chromosome 12q15. The expression of BEST3 was predominantly observed in skeletal muscle and weaker in brain, spinal cord, bone marrow, retina, thymus, and testis (PMID: 12032738). Specification Tested Reactivity: human, mouse Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14255 Product name: Recombinant human BEST3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 483-668 aa of BC028087 Sequence: SLTPQSSVRTSPIKMPLVPEVLITAAEAPVPTSGGYHHDSATSILSSEFTGVQPSKTEQQQGPMGSILSPSEKETPPGGPSPQTVSASAEENIFNCEEDPGDTFLKRWSLPGFLGSSHTSLGNLSPDPMSSQPALLIDTETSSEISGINIVAGSRVSSDMLYLMENLDTKETDIIELNKETEESPK Predict reactive species Full Name: bestrophin 3 Calculated Molecular Weight: 668 aa, 76 kDa Observed Molecular Weight: 76-78 kDa, 50-55 kDa GenBank Accession Number: BC028087 Gene Symbol: BEST3 Gene ID (NCBI): 144453 RRID: AB_10666435 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N1M1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.