Proteintech, 20466-1-AP, INF2 Polyclonal antibody

Size: 20ul / 150ul The INF2 (20466-1-AP) by Proteintech is a Polyclonal antibody targeting INF2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 20466-1-AP targets INF2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A431 cells, HepG2 cells, NIH/3T3 cells, HeLa cells, PC-3 cells, K-562 cells Positive IP detected in: NIH/3T3 cells Positive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information INF2 is a member of the formin family of actin-regulating proteins. INF2 is peripherally bound to the cytoplasmic face of the endoplasmic reticulum (ER). Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis. The predicted MW of INF2 is around 136 kDa, while higher molecular mass from 140-200 kDa has been reported in literature (26383224,20493814,19366733). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14302 Product name: Recombinant human INF2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC008756 Sequence: MVRPKAQLVLAACESLLTSRQLPIFCQLILRIGNFLNYGSHTGDADGFKISTLLKLTETKSQQNRVTLLHHVLEEAEKSHPDLLQLPRDLEQPSQAAGINLEIIRSEASSNLKKLLETERKVSASVAEVQEQYTERLQASISAFRALDELFEAIEQKQRELADYLCEDAQQLSLEDTFSTMKAFRDLFLRALKENKDRKEQAAKAERRKQQLAEEEARRPRGEDGKPVRKGPGKQEEVCVIDALLADIRKGFQLRKTARGRGDTDGGSKAASMDPPRATEPVATSNPAGDPVGSTRCPASEPGLDATTASESRGWDLVDAVTPGPQPTLEQLEEGGPRPLERRSSWYVDA Predict reactive species Full Name: inverted formin, FH2 and WH2 domain containing Calculated Molecular Weight: 1249 aa, 136 kDa Observed Molecular Weight: 180-200 kDa GenBank Accession Number: BC008756 Gene Symbol: INF2 Gene ID (NCBI): 64423 RRID: AB_10694821 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q27J81 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.