Proteintech, 20561-1-AP, Synaptotagmin-14 Polyclonal antibody

Size: 20ul / 150ul The Synaptotagmin-14 (20561-1-AP) by Proteintech is a Polyclonal antibody targeting Synaptotagmin-14 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 20561-1-AP targets Synaptotagmin-14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IHC detected in: mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:150-1:600 Background Information The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-14 (SYT14) is highly expressed in brain tissues. A homozygous mutation of SYT14 is involved in the pathogenesis of spinocerebellar ataxia (SCA) (PMID: 21835308). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: synaptotagmin XIV Calculated Molecular Weight: 62 kDa Observed Molecular Weight: 62-65 kDa GenBank Accession Number: NM_153262 Gene Symbol: Synaptotagmin-14 Gene ID (NCBI): 255928 RRID: AB_3085607 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8NB59 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.