Proteintech, 20566-1-AP, RIG-1/DDX58 Polyclonal antibody

Size: 20ul / 150ul The RIG-1/DDX58 (20566-1-AP) by Proteintech is a Polyclonal antibody targeting RIG-1/DDX58 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 20566-1-AP targets RIG-1/DDX58 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, A431 cells, MCF-7 cells, mouse brain tissue, HepG2 cells, MOLT-4 cells Positive IP detected in: HepG2 cells Positive IF/ICC detected in: H9C2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, pig, monkey, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 Calculated Molecular Weight: 106 kDa Observed Molecular Weight: 101~106 kDa GenBank Accession Number: NM_014314 Gene Symbol: RIG-1/DDX58 Gene ID (NCBI): 23586 RRID: AB_10700006 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95786 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.