Proteintech, 20569-1-AP, Peroxiredoxin 2 Polyclonal antibody

Size: 20ul / 150ul The Peroxiredoxin 2 (20569-1-AP) by Proteintech is a Polyclonal antibody targeting Peroxiredoxin 2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 20569-1-AP targets Peroxiredoxin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells Positive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:150-1:600 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information PRDX2, also named as NKEFB, TDPX1, TSA and PRP, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. PRDX2 may play an important role in eliminating peroxides generated during metabolism. It might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. The antibody is specific to PRDX2. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: peroxiredoxin 2 Calculated Molecular Weight: 22 kDa Observed Molecular Weight: 22 kDa GenBank Accession Number: NM_005809 Gene Symbol: peroxiredoxin 2 Gene ID (NCBI): 7001 RRID: AB_10695186 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P32119 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.