Proteintech, 20576-1-AP, CHD1 Polyclonal antibody

Size: 20ul / 150ul The CHD1 (20576-1-AP) by Proteintech is a Polyclonal antibody targeting CHD1 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 20576-1-AP targets CHD1 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, A431 cells, human brain tissue Positive IP detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:300-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information CHD1 belongs to the SNF2/RAD54 helicase family. CHD1 is an aTP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. CHD1 regulates polymerase II transcription. It is required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. CHD1 regulates negatively DNA replication. It is required to maintain a specific chromatin configuration across the genome. It is associated with histone deacetylase (HDAC) activity. CHD1 is required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. It functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. It is required for maintaining open chromatin and pluripotency in embryonic stem cells. In PTEN-deficient prostate and breast cancers, CHD1 depletion profoundly and specifically suppressed cell proliferation, cell survival and tumorigenic potential. The antibody is specific to CHD1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: chromodomain helicase DNA binding protein 1 Calculated Molecular Weight: 197 kDa Observed Molecular Weight: 200 kDa GenBank Accession Number: NM_001270 Gene Symbol: CHD1 Gene ID (NCBI): 1105 RRID: AB_10694288 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O14646 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.