Proteintech, 20661-1-AP, MARCKS Polyclonal antibody

Size: 20ul / 150ul The MARCKS (20661-1-AP) by Proteintech is a Polyclonal antibody targeting MARCKS in WB, IHC, IF-P, IP, ELISA applications with reactivity to human, mouse, rat samples 20661-1-AP targets MARCKS in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, IMR-32 cells, mouse brain tissue, rat brain tissue, SH-SY5Y cells, SK-N-SH cells Positive IP detected in: A431 cells Positive IHC detected in: human normal colonNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information MARCKS, also named as MACS, PRKCSL and 80K-L protein, belongs to the MARCKS family. MARCKS is the most prominent cellular substrate for protein kinase C. It binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: myristoylated alanine-rich protein kinase C substrate Calculated Molecular Weight: 32 kDa Observed Molecular Weight: 80 kDa GenBank Accession Number: NM_002356 Gene Symbol: MARCKS Gene ID (NCBI): 4082 RRID: AB_2878719 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P29966 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.