Proteintech, 20671-1-AP, Synaptotagmin-8 Polyclonal antibody

Size: 20ul / 150ul The Synaptotagmin-8 (20671-1-AP) by Proteintech is a Polyclonal antibody targeting Synaptotagmin-8 in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 20671-1-AP targets Synaptotagmin-8 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: human placenta tissue, mouse placenta tissue Positive IF/ICC detected in: Caco-2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT8 (synaptotagmin-8) is related to insulin secretion, and its expression is positively regulated by the insulin promoter (PMID: 21336277). Due to alternative splicing, SYT8 may produce proteins with molecular weights between 25 and 40 to 44 kDa. An additional band of 50 kDa could also be detected (PMID: 16386321) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: synaptotagmin VIII Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 40 kDa, 50 kDa GenBank Accession Number: NM_138567 Gene Symbol: SYT8 Gene ID (NCBI): 90019 RRID: AB_3085610 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8NBV8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.