Proteintech, 20678-1-AP, LIS1 Polyclonal antibody

Size: 20ul / 150ul The LIS1 (20678-1-AP) by Proteintech is a Polyclonal antibody targeting LIS1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 20678-1-AP targets LIS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Platelet-activating factor acetylhydrolase 1B subunit alpha (LIS1), a critical mediator of neuronal migration in developing brain, is expressed throughout life. LIS1 forms a homodimer that interacts directly with the dynein complex and is essential for its enrichment at dynamic microtubule plus ends in mammalian cells. In addition to regulating the association of dynein with microtubule plus ends, LIS1 is required for efficient transport of many dynein-associated cargos and proper localization of Golgi complexes, endosomes, lysosomes, and mitochondria. The LIS1 protein has a calculated molecular mass of ~45 kDa.(PMID: 29150182, PMID: 29562183, PMID: 15800059) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa Calculated Molecular Weight: 47 kDa Observed Molecular Weight: 45-50 kDa GenBank Accession Number: NM_000430 Gene Symbol: LIS1 Gene ID (NCBI): 5048 RRID: AB_2878723 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P43034 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.