Proteintech, 20697-1-AP, SHH Polyclonal antibody

Size: 20ul / 150ul The SHH (20697-1-AP) by Proteintech is a Polyclonal antibody targeting SHH in WB, IHC, IF, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 20697-1-AP targets SHH in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, rat kidney tissue, HepG2 cells, LNCaP cells, PC-3 cells, mouse liver tissue, rat liver tissue Positive IP detected in: mouse liver tissue Positive IHC detected in: human liver cancer tissue, mouse kidney tissue, human pancreas cancer tissue, human ovary tumor tissue, mouse embryo tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF detected in: mouse kidney tissue Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF): IF : 1:10-1:100 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Sonic hedgehog (SHH) is a member of the Hedgehog (Hh) family of proteins that control cell growth,survival,and fate.What is the molecular weight of SHH?The molecular weight of SHH is approximately 45 kDa, but it is cleaved autocatalytically to generatea 20 kDaN-terminal fragment and a 25 kDa C-terminal fragment responsible for hedgehogbiologicalactivity andtheauto-processing machinery respectively (PMID: 10753901).What is the tissue specificity of SHH?SHH is the most broadly expressed mammalian Hh signaling molecule. During early vertebrate development,SHH is expressed in midline tissues such as the node, notochord, and floor plate to controlthe left-right anddorso-ventral patterning of the embryo (PMID: 12729563). SHH is expressed in fetal intestine, liver, kidney,and lung tissue, but is not expressed in adult tissues.What is the cellular localization of SHH?SHH is localized to the cell membrane. The N-terminal fragment is associated with lipid rafts atthe cellsurface,whereas the C-terminal fragment diffuses from the cell.What is the role of SHH in the Sonic hedgehog signaling pathway?This pathway is one of the major trafficking networks that regulate key events during developmental growthand patterning (PMID: 19368798). Loss of SHH signaling and regulation has been implicated in birth defects,tissue regeneration, stem cell renewal, and cancer growth (PMID: 15549094). The regulatory action of the SHHpathway is linked to the secretion, uptake, and translocation of the SHH protein; a ligand precursor, which isimplicated in the regulation of the central nervous system polarity and neural patterning (PMID: 11130178,11801325). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, mink Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: sonic hedgehog homolog (Drosophila) Calculated Molecular Weight: 50 kDa Observed Molecular Weight: 45-51 kDa GenBank Accession Number: NM_000193 Gene Symbol: SHH Gene ID (NCBI): 6469 RRID: AB_10694828 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15465 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.