Proteintech, 20702-1-AP, IRS2 Polyclonal antibody

Size: 20ul / 150ul The IRS2 (20702-1-AP) by Proteintech is a Polyclonal antibody targeting IRS2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 20702-1-AP targets IRS2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IHC detected in: mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The family of INS receptor substrates (IRS) consists of four proteins (IRS-1-IRS-4), which were initially characterized as typical cytosolic adaptor proteins involved in INS receptor (IR) and INS-like growth factor I receptor (IGF-IR) signaling. Impaired glucose tolerance and INS resistance, and thus type 2 diabetes were demonstrated in Irs2 knockout mice. In vivo data also indicated that he regulation of beta-cell mass involves IRS2, while IRS1 is only required for proper INS production in beta-cells. IRS2, like IRS1, has a predicted molecular weight of 137 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS-PAGE as a band of approximately 150-185 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: IRS2 Calculated Molecular Weight: 137 kDa Observed Molecular Weight: 150-185 kDa GenBank Accession Number: NM_003749 Gene Symbol: IRS2 Gene ID (NCBI): 8660 RRID: AB_2918067 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y4H2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.