Proteintech, 20705-1-AP, TOP1 Polyclonal antibody

Size: 20ul / 150ul The TOP1 (20705-1-AP) by Proteintech is a Polyclonal antibody targeting TOP1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 20705-1-AP targets TOP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: L02 cells Positive IHC detected in: human stomach cancer tissue, human colon cancer tissue, human cervical cancer tissue, human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200 Background Information TOP1 belongs to the eukaryotic type I topoisomerase family. The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. The antibody is specific to TOP1. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: topoisomerase (DNA) I Calculated Molecular Weight: 91 kDa Observed Molecular Weight: 90 kDa GenBank Accession Number: NM_003286 Gene Symbol: TOP1 Gene ID (NCBI): 7150 RRID: AB_10695762 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P11387 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.