Proteintech, 20729-1-AP, ITPR3 Polyclonal antibody

Size: 20ul / 150ul The ITPR3 (20729-1-AP) by Proteintech is a Polyclonal antibody targeting ITPR3 in WB, IHC, ELISA applications with reactivity to human, mouse samples 20729-1-AP targets ITPR3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells Positive IHC detected in: mouse colon tissue, mouse cerebellum tissue, mouse small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information ITPRs family have three members: ITPR1, ITPR2 and ITPR3. For ITPR3, it is associated with cervical squamous cell carcinoma, glioblastoma, cholangiocarcinoma and other cancers. Inositol 1,4, 5-triphosphate receptor type 3 (ITPR3) was found to be apical-located in bile duct cells, and disruption of lipid raft in the segregated bile duct unit (IBDU) redistributes ITPR3 and impairs Ca2+ waves. (PMID: 35580861, PMID:35852334) Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: inositol 1,4,5-triphosphate receptor, type 3 Calculated Molecular Weight: 304 kDa Observed Molecular Weight: 250-300 kDa GenBank Accession Number: NM_002224 Gene Symbol: ITPR3 Gene ID (NCBI): 3710 RRID: AB_3669343 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14573 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.