Proteintech, 20962-1-AP, PYCR1-specific Polyclonal antibody

Size: 20ul / 150ul The PYCR1-specific (20962-1-AP) by Proteintech is a Polyclonal antibody targeting PYCR1-specific in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 20962-1-AP targets PYCR1-specific in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Raji cells, FaDu cells, human brain tissue, HepG2 cells, MCF-7 cells, Ramos cells, U2OS cells Positive IP detected in: Raji cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35kd. This antibody is specific to PYCR1. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: pyrroline-5-carboxylate reductase 1 Calculated Molecular Weight: 33 kDa Observed Molecular Weight: 33-35 kDa GenBank Accession Number: NM_006907 Gene Symbol: PYCR1 Gene ID (NCBI): 5831 RRID: AB_10638486 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P32322 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.