Proteintech, 21207-1-AP, TET2 Polyclonal antibody

Size: 20ul / 150ul The TET2 (21207-1-AP) by Proteintech is a Polyclonal antibody targeting TET2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse samples 21207-1-AP targets TET2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, RIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A431 cells, HL-60 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells Positive IP detected in: mouse brain tissue Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells, RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information TET2 is an enzyme which converts 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) in DNA and is important for normal myelopoiesis. TET2 mutations are frequently observed in myeloid malignancies, including myeloproliferative neoplasms. TET2 is broadly expressed in a variety of tissues. In healthy tissues, TET2 expression was shown to be elevated in hematopoietic cells with highest expression in granulocytes. Three isoforms of TET2 exists due to the alternative splicing. 220-250 kDa bands represent the full-length isoform; 130-133 kDa bands represent the nuclear isoforms of TET2. (PMID: 24615693) This antibody recognizes all three isoforms of TET2. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, goat, sheep Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15583 Product name: Recombinant human TET2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC150180 Sequence: MEQDRTNHVEGNRLSPFLIPSPPICQTERLATKLQNGSPLPERAHPEVNGDTKWHSFKSYYGIPCMKGSQNSRVSPDFTQESRGYSKCLQNGGIKRTVSEPSLSGLLQIKKLKQDQKANGERRNFGVSQERNPGESSQPNVSDLSDKKESVSSVAQENAVKDFTSFSTHNCSGPENPELQILNEQEGKSANYHDKNIVLLKNKAVLMPNGATVSASSVEHTHGELLEKTLSQYYPDCVSIAVQKTTSHINAINSQATNELSCEITHPSHTSGQINSAQTSNSELPPKPAAVVSEACDADDADNASKLAAMLNTCSFQKPEQLQQQKSVFEICPSPAENNIQGTTKLASGE Predict reactive species Full Name: tet oncogene family member 2 Calculated Molecular Weight: 2002 aa, 224 kDa Observed Molecular Weight: 130 kDa, 250 kDa GenBank Accession Number: BC150180 Gene Symbol: TET2 Gene ID (NCBI): 54790 RRID: AB_10734584 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6N021 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.