Proteintech, 21299-1-AP, PLK5 Polyclonal antibody

Size: 20ul / 150ul The PLK5 (21299-1-AP) by Proteintech is a Polyclonal antibody targeting PLK5 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 21299-1-AP targets PLK5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: K-562 cells, mouse testis tissue, mouse kidney tissue Positive IHC detected in: human brain tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information PLK5, also named as PLK5P, is an inactive serine/threonine-protein kinase that plays a role in cell cycle progression and neuronal differentiation. Compared with mouse Plk5, the protein kinase domain of human PLK5 is truncated. This is the reason that the MW of mouse Plk5 is about 50-70 kDa, the MW of human PLK5 is about 32-40 kDa. PLK5 is located in the cytoplasm and nucleus(Uniprot). This antibody detects both PLK5 gene of human and mouse. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15881 Product name: Recombinant human PLK5P protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-336 aa of BC100792 Sequence: MYTVLTGTPPFMASPLSEMYQNIREGHYPEPAHLSANARRLIVHLLAPNPAERPSLDHLLQDDFFTQGFTPDRLPAHSCHSPPIFAIPPPLGRIFRKVGQRLLTQCRPPCPFTPKEASGPGEGGPDPDSMEWDGESSLSAKEVPCLEGPIHLVAQGTLQSDLAGPEGSRRPEVEAALRHLQLCLDVGPPATQDPLGEQQPILWAPKWVDYSSKYGFGYQLLDGGRTGRHPHGPATPRREGTLPTPVPPAGPGLCLLRFLASEHALLLLFSNGMVQVSFSGVPAQLVLSGEGEGLQLTLWEQGSPGTSYSLDVPQSHGCAPTTGQHLHHALRMLQSI Predict reactive species Full Name: polo-like kinase 5 pseudogene Calculated Molecular Weight: 336 aa, 36 kDa Observed Molecular Weight: 55-60 kDa GenBank Accession Number: BC100792 Gene Symbol: PLK5 Gene ID (NCBI): 126520 RRID: AB_10732958 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q496M5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.