Proteintech, 21308-1-AP, EMILIN3 Polyclonal antibody

Size: 20ul / 150ul The EMILIN3 (21308-1-AP) by Proteintech is a Polyclonal antibody targeting EMILIN3 in IHC, IP, ELISA applications with reactivity to human samples 21308-1-AP targets EMILIN3 in IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive IP detected in: COLO 320 cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information EMILIN3 (Elastin Microfibril Interfacer 3) is a protein-coding gene that encodes an extracellular matrix glycoprotein. It is a member of the EMILIN family and plays a role in the organization of the extracellular matrix, particularly in elastic fiber formation and extracellular matrix organization. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15925 Product name: Recombinant human EMILIN3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 418-766 aa of BC101045 Sequence: DELTRLSAAMLEGGVDGLLEGLETLNGTEGGARGCCLRLDMGGWGVGGFGTMLEERVQSLEERLATLAGELSHDSASPGRSARPLVQTELAVLEQRLVSLETSCTPSTTSAILDSLVAEVKAWQSRSEALLRQVASHAALLQQLNGTVAEVQGQLAEGTGSSLQGEITLLKVNLNSVSKSLTGLSDSVSQYSDAFLAANTSLDERERKVEAEVQAIQEQVSSQGSRLQAGHRQVLNLRGELEQLKAGVAKVASGLSRCQDTAQKLQHTVGHFDQRVAQVEGACRRLGLLAAGLDSLPTEPLRPREGLWSHVDQLNRTLAQHTQDIARLRDDLLDCQAQLAEQVRPGQAN Predict reactive species Full Name: elastin microfibril interfacer 3 Calculated Molecular Weight: 766 aa, 83 kDa Observed Molecular Weight: 83 kDa GenBank Accession Number: BC101045 Gene Symbol: EMILIN3 Gene ID (NCBI): 90187 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NT22 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.