Proteintech, 21349-1-AP, TRPC4 Polyclonal antibody

Size: 20ul / 150ul The TRPC4 (21349-1-AP) by Proteintech is a Polyclonal antibody targeting TRPC4 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 21349-1-AP targets TRPC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, mouse brain tissue, rat brain tissue Positive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information TRPC4 (Transient Receptor Potential Paradigm 4) is a non-selective calcium channel protein widely found in the nervous system and cardiovascular system. TRPC4 regulates intracellular calcium levels through the activation of signaling pathways mediated by Gq/11 and Gi/o-coupled receptors, and is involved in neurotransmission, neuronal excitability, and vascular endothelial cell function. In the nervous system, TRPC4 is associated with neuropsychiatric disorders such as depression and anxiety, and its inhibitors show antidepressant and anxiolytic potential. In the cardiovascular system, TRPC4 affects vascular permeability and diastolic function by regulating calcium ion inward flow and plays an important role in hypoxia-induced pulmonary hypertension. In addition, TRPC4 is involved in thermoregulation, sensing changes in internal temperature to regulate the drop in body temperature. aberrant expression of TRPC4 is associated with a variety of diseases, making it a potential therapeutic target! Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15698 Product name: Recombinant human TRPC4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 746-977 aa of BC104725 Sequence: FRFEVLGLLRGSKLSTIQSANASKESSNSADSDEKSDSEGNSKDKKKNFSLFDLTTLIHPRSAAIASERHNISNGSALVVQEPPREKQRKVNFVTDIKNFGLFHRRSKQNAAEQNANQIFSVSEEVARQQAAGPLERNIQLESRGLASRGDLSIPGLSEQCVLVDHRERNTDTLGLQVGKRVCPFKSEKVVVEDTVPIIPKEKHAKEEDSSIDYDLNLPDTVTHEDYVTTRL Predict reactive species Full Name: transient receptor potential cation channel, subfamily C, member 4 Calculated Molecular Weight: 977 aa, 112 kDa Observed Molecular Weight: 100-120 kDa GenBank Accession Number: BC104725 Gene Symbol: TRPC4 Gene ID (NCBI): 7223 RRID: AB_10734426 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UBN4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.