Proteintech, 21369-1-AP, Cingulin Polyclonal antibody

Size: 20ul / 150ul The Cingulin (21369-1-AP) by Proteintech is a Polyclonal antibody targeting Cingulin in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 21369-1-AP targets Cingulin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293T cells, mouse kidney tissue, mouse small intestine tissue, MCF-7 cells Positive IP detected in: mouse kidney tissue Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:400-1:1600 Background Information Cingulin is a component of the cytoplasmic plaque of tight junction, and is localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Cingulin is a homodimer, each subunit containing a globular "head" domain, a coiled-coil "rod" domain and a small globular "tail" region. Cingulin can interact interact with ZO-1, ZO-2, ZO-3, actin, and myosin. It probably plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, canine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15843 Product name: Recombinant human CGN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC146657 Sequence: MAEPRGPVDHGVQIRFITEPVSGAEMGTLRRGGRRPAKDARASTYGVAVRVQGIAGQPFVVLNSGEKGGDSFGVQIKGANDQGASGALSSDLELPENPYSQVKGFPAPSQSSTSDEEPGAYWNGKLLRSHSQASLAGPGPVDPSNRSNSMLELAPKVASPGSTIDTAPLSSVDSLINKFDSQLGGQARGRTGRRTRMLPPEQRKRSKSLDSRLPRDTFEERERQSTNHWTSSTKYDNHVGTSKQPAQSQNLSPLSGFSRSRQTQDWVLQSFEEPRRSAQDPTMLQFKSTPDLLRDQQEAAPPGSVDHMKATIYGILREGSSESETSVRRKVSLVLEKMQPLVMVSSGSTK Predict reactive species Full Name: cingulin Calculated Molecular Weight: 1197 aa, 136 kDa Observed Molecular Weight: 136-150 kDa GenBank Accession Number: BC146657 Gene Symbol: Cingulin Gene ID (NCBI): 57530 RRID: AB_10733647 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9P2M7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.