Proteintech, 21377-1-AP, SASS6 Polyclonal antibody

Size: 20ul / 150ul The SASS6 (21377-1-AP) by Proteintech is a Polyclonal antibody targeting SASS6 in WB, IHC, IP, ELISA applications with reactivity to human samples 21377-1-AP targets SASS6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, human testis tissue, MCF-7 cells Positive IP detected in: HeLa cells Positive IHC detected in: mouse testis tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Spindle assembly abnormal protein 6 homolog (SASS6) is a centrosomal protein that, together with PLK4, CEP135, CENPJ (also known as CPAP) and STIL, is involved in regulating the number of centrosomes in human cells. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15917 Product name: Recombinant human SASS6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 141-490 aa of BC101026 Sequence: VECHEKEKHVNQLQTKVAVLEQEIKDKDQLVLRTKEAFDTIQEQKVVLEENGEKNQVQLGKLEATIKSLSAELLKANEIIKKLQGDLKTLMGKLKLKNTVTIQQEKLLAEKEEKLQKEQKELQDVGQSLRIKEQEVCKLQEQLEATVKKLEESKQLLKNNEKLITWLNKELNENQLVRKQDVLGPSTTPPAHSSSNTIRSGISPNLNVVDGRLTYPTCGIGYPVSSAFAFQNTFPHSISAKNTSHPGSGTKVQFNLQFTKPNASLGDVQSGATISMPCSTDKENGENVGLESKYLKKREDSIPLRGLSQNLFSNSDHQRDGTLGALHTSSKPTALPSASSAYFPGQLPNS Predict reactive species Full Name: spindle assembly 6 homolog (C. elegans) Calculated Molecular Weight: 657 aa, 74 kDa Observed Molecular Weight: 70-74 kDa GenBank Accession Number: BC101026 Gene Symbol: SASS6 Gene ID (NCBI): 163786 RRID: AB_2878851 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6UVJ0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.