Proteintech, 21448-1-AP, PVRIG Polyclonal antibody

Size: 20ul / 150ul The PVRIG (21448-1-AP) by Proteintech is a Polyclonal antibody targeting PVRIG in WB, IHC, ELISA applications with reactivity to human samples 21448-1-AP targets PVRIG in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MOLT-4 cells, NK-92 cells, Jurkat cells Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information PVRIG, also named as CD112R and C7orf15, is an immune suppressive molecule expressed on T and NK cells. It's a cell surface receptor for NECTIN2. PVRIG is a target for the treatment of cancer. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13815 Product name: Recombinant human PVRIG protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 193-326 aa of BC001129 Sequence: LRRHKHRPAPRLQPSRTSPQAPRARAWAPSQASQAALHVPYATINTSCRPATLDTAHPHGGPSWWASLPTHAAHRPQGPAAWASTPIPARGSFVSVENGLYAQAGERPPHTGPGLTLFPDPRGPRAMEGPLGVR Predict reactive species Full Name: poliovirus receptor related immunoglobulin domain containing Calculated Molecular Weight: 326 aa, 34 kDa Observed Molecular Weight: ~34 kDa GenBank Accession Number: BC001129 Gene Symbol: PVRIG Gene ID (NCBI): 79037 RRID: AB_2878859 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6DKI7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.